proteome profiler human angiogenesis antibody array kit  (R&D Systems)

Journal: bioRxiv

Article Title: hsa-miR-9-5p highly expressed in syncytiotrophoblast-derived extracellular vesicles from early-onset preeclampsia impairs cerebral microvascular endothelial cell pro-angiogenic capacity

doi: 10.1101/2024.10.21.619546

Figure Lengend Snippet: A) Representative image of the Proteome Profiler™ Human Angiogenesis Antibody Array in hCMEC/D3 treated (48 h) with mirVana™ miRNA Mimic, Negative Control (Scramble), or hsa-miR-9-5p (10 nM). Left image densitometry of the representative blots. Right images represent ImageJ conversion in arbitrary color scale (white to blue, representing low and high expression respectively). B) Log2 fold of change of differentially expressed proteins. Scatter line represent 0.5 Log fold of change. C) Protein-protein interaction (PPI) network construction and cluster analysis as described in methods section. D) VCAM-1 protein levels in cell culture supernatants of hCMEC/D3 after stimulation with tumor necrosis factor-α (TNF-α, of 10 ng/mL for 16 hours) in presence of hsa-miR-9-5p (10 nM). The control groups received an equivalent volume of fresh supplemented EGM-2 growth medium with and without scramble construct. VCAM-1: vascular cell adhesion molecule 1, NS, non-significant differences. ** p <0.01. *** p <0.001; **** p <0.0001.

Article Snippet: The expression patterns of proteins associated with angiogenesis were assessed utilizing the Proteome Profiler™ Human Angiogenesis Antibody Array Kit (R&D Systems, Minneapolis, MN, USA).

Techniques: Ab Array, Negative Control, Expressing, Cell Culture, Control, Construct

Journal: International Journal of Molecular Sciences

Article Title: PP2A Affects Angiogenesis via Its Interaction with a Novel Phosphorylation Site of TSP1

doi: 10.3390/ijms25031844

Figure Lengend Snippet: B55α depletion affects TSP1 in endothelial cells. ( A ) Cell lysates of non-targeting siRNA and B55α-specific siRNA (siPPP2R2A)-treated BPAEC were incubated on angiogenetic-specific antibodies containing proteome profiler array membrane. Quantification of the dots was performed using the ImageJ software (version 1.54h). Bars represent the mean of duplicated dots ± SD. Statistical analysis was performed by unpaired t-test (* p < 0.05, ** p < 0.01, and *** p < 0.001). ( B ) Control, non-targeting, and B55α-specific siRNA-treated BPAEC lysates were tested by Western blot. Quantitative analysis was performed by densitometry of the blots using actin bands for normalization. Statistical analysis was performed using ANOVA Tukey’s test (n = 3, *** p < 0.001). ( C ) qPCR measurements were made using mRNA from nonsiRNA and B55α-depleted (siPPP2R2A) BPAEC cells. Quantitative analysis of B55α and TSP1 signals are shown. GAPDH was used for mRNA-level normalization. Significant differences were determined by unpaired t-test (n = 3, *** p < 0.001).

Article Snippet: Proteome Profiler Human Angiogenesis Antibody Array (ARY007) was ordered from R&D Systems, Inc. (Minneapolis, MN, USA).

Techniques: Incubation, Membrane, Software, Control, Western Blot